Table 1.

O-GlcNAc sites identified following labelling with 2, Neutravidin affinity purification, and hydrazine mediated elution. Sites and regions of modification are denoted in red or red underline, respectively. Novel site identifications are marked by an asterisk.

Protein	Peptide Sequence	Site(s)	Mascot Ion Score	Mascot Delta Ion Score	Method
α-crystallin A	AIPVSREEKPSSAPSS	Ser-162	24.9	23.5	ETD
sOGT	ISPTFADAYSNMGNTLK	Ser-10*/Thr-12*	46.5	-	ETD
sOGT	ISPTFADAYSNMGNTLK	Ser-20*	21.6	13.6	ETD
sOGT	ISPTFADAYSNMGNTLK	Ser-10*/Thr- 12*, Ser-20*	38.4	-	ETD
sOGT	EMQDVQGALQCYTR	Thr-38	41.8	35.0	CID
sOGT	AIQINPAFADAHSNLASIHKDSGNIPEAIASYR	Ser-52*	53.5	7.9	ETD
sOGT	AIQINPAFADAHSNLASIHKDSGNIPEAIASYR	Ser-56*	56.8	15.7	ETD
sOGT	LYLQMWEHYAAGNKPDHMIKPVEVTESA	Thr-662	33.1	8.0	ETD