Fig. 4.

IL-2, IL-4, IFN-γ, or TNF-α reinforces hsBAFF-induced activation of Erk1/2 and S6K1 pathways in B cells. Raji cells were treated with hsBAFF (0–0.25 μg/ml) for 12 h, or with/without hsBAFF (0.25 μg/ml) in the presence or absence of IL-2 (5 and 50 ng/ml), IL-4 (5 and 25 ng/ml), IFN-γ (10 and 100 ng/ml), or TNF-α (5 and 50 ng/ml) for 12 h. (A and C) Total cell lysates were subjected to Western blotting using indicated antibodies. The blots were probed for β-actin as a loading control. Similar results were observed in at least three independent experiments (A and C), and the blots for p-Erk1/2, p-S6K1, and p-S6 were semi-quantified (B and D). Results are presented as mean ± S.E. (n = 3). ^ap<0.05, difference with control group. ^bp<0.05, difference with 0.25 μg/ml hsBAFF group.