Skip to main content
. Author manuscript; available in PMC: 2016 Jun 13.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2016 Feb 24;51(3):162–184. doi: 10.3109/10409238.2016.1143913

Figure 7.

Figure 7

Cdc6 is necessary for assembly of the pre-replication complex and subsequent DNA synthesis. In G0, Cdc6 is ubiquinated by the anaphase promoting complex/cyclosome (APC/C) and degraded by the proteasome. In G1, Cyclin E/CDK2 phosphorylates Cdc6 on S54 and S74, protecting it from degradation. Cdc6 is translocated into the nucleus where it binds the origin recognition complex and is required to recruit Cdt1 and MCM2–7 and form the prereplication complex. After firing of the origins, Cdc6 is methylated by GCN5 causing its dissociation from the ORC. Cdc6 is then phosphorylated on S106 by Cyclin A/CDK2 and translocated to the cytoplasm. PP2A-PR70 is thought to dephosphorylate Cdc6 either at this point in G2 and/or in G1, ensuring Cdc6 destruction and regulating DNA synthesis. Figure adapted from Mumby (2009). (see colour version of this figure at www.informahealthcare.com/bmg).