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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Dev Biol. 2016 Apr 27;415(1):87–97. doi: 10.1016/j.ydbio.2016.04.018

Figure 2. In the absence of grim and rpr ventral muscle fiber number is doubled.

Figure 2

A–E”’) Abdominal filet preps stained with phalloidin (red) to mark muscles, and Hoechst 33342 (green) to mark DNA. Fiber numbers were quantified by counting columns of nuclei, as shown in A”-E”, and illustrated with dashed lines in A”’-E”’. Heterozygotes for the grim rpr double mutant (3.5A) and the grim to rpr deletion (MM2) have an average of 10 fibers/segment. When grim and rpr are completely removed in 3.5A/MM2, fiber number is dramatically increased to about 15–20 fibers/segment (D–D”’, F). Adults were dissected within 2 days of eclosion, and many retain distinctive larval muscles (arrow in A). In both heterozygous and homozygous grim rpr mutants we frequently noted transverse ventral muscle fibers (arrow in E’), possibly due to the retention of large larval epidermal cells at the ventral midline (arrow in E”). F) Quantification of average number of fibers per segment. Three segments per abdomen (A3-A5) were counted (x̄ shown as bar.) Animals/genotype yw n=5, MM2/+ n=8, 3.5A/+ n=8, 3.5A/MM2 n=10. Images were either taken at a magnification of 10X (A–E) or 60X (A’-B’).