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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cell Biochem Biophys. 2016 Jun;74(2):263–276. doi: 10.1007/s12013-015-0715-4

FIGURE 1.

FIGURE 1

Kv1.2 and Kv1.5 are expressed at the RNA and protein level in isolated smooth muscle cells from SMA. A: Ethidium bromide stained agarose gel of cPCR products for Kv1.2 and Kv1.5 in RNA from freshly dispersed tail (TA) and small mesenteric arteries (SMA) cells. First strand products from reactions performed in the presence (+) or absence (−) of reverse transcriptase were used as templates for PCR. Brain RNA (B) was used as a positive control for cDNA synthesis while genomic DNA (G) and water (W) were used as PCR templates for positive and negative PCR controls. B: Immunofluorescence detection of Kv1.2 and Kv1.5 protein in freshly dispersed SMA myocytes.