Skip to main content
. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cell Biochem Biophys. 2016 Jun;74(2):263–276. doi: 10.1007/s12013-015-0715-4

FIGURE 9.

FIGURE 9

Kv4 channels are not functionally expressed in SMA. A: Agarose gels showing expression of Kv4.1 and 4.3 in cellular RNA from TA and SMA by cPCR. B: Western blot suggesting expression of Kv4.1 and 4.3 in tissues as well as rat brain. C: Immunofluorescence showed no apparent plasma membrane expression of Kv4.1 or Kv4.3 in SMA myocytes. D: Neither heteropodotoxin (HpTx, 100 nmol/L) nor phrixotoxin (PhTx, 1 μmol/L) had an effect on whole cell Kv currents in SMA myocytes. Voltage clamp protocols are as described in Figure 2. Horizontal and vertical calibration bars represent 300 pA for HpTx and 120 pA for PhTx, and 200 msec, respectively. E: Heteropodotoxin had no effect on wall stress (% of KCl) in SMA precontracted with 30 nmol/L U46619. Horizontal bar is 2 min.