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. 2016 Jun 13;11(6):e0157435. doi: 10.1371/journal.pone.0157435

Fig 1. Schematic representation of the FMDV genome and the rSFV plasmids used in this study.

Fig 1

The P1-2A, P1-2A-3CC142S and P1-2A-mIRES-3C FMDV cDNA cassettes have been described elsewhere [13,26] while the pSFV3 and the split helper plasmids have also been described previously [23]. Positions of relevant restriction enzyme sites used are shown. Abbreviations: P1-2A: capsid precursor protein; 3C: 3Cpro wild-type or C142S mutant; mIRES: internal ribosome entry site GTTA mutant; SP6: SP6 promotor; nsP1-P4: SFV non-structural proteins 1–4; PS: packaging signal; 26S: SFV 26S subgenomic promotor; C: SFV capsid; p62, 6K and E1: SFV spike proteins.