Fig 3. Characterization of expressed FMDV capsid proteins.
(A) Uninfected BHK cells (control, lane 1) or cells infected with rSFV (lane 2), rSFV-FMDV-P1-2A (lane 3), rSFV-FMDV-P1-2A-3CC142S (lane 4) or rSFV-FMDV-P1-2A-mIRES-3C (lane 5) were harvested and the cell lysates were fractionated by SDS–PAGE. The proteins were transferred to PVDF membrane and probed with antibodies specific for FMDV capsid proteins (top), FMDV 2A (second), FMDV 3Cpro (third) and β-actin (bottom) as indicated. Detection of β-actin was used as a control for equal protein loading. The results shown are representative of three independent experiments. Molecular mass markers (kDa) are indicated on the left. (B) Cell lysates, as used in panel A, were diluted (10-fold initially and then 2-fold dilutions) and analysed with a FMDV serotype O-specific antigen ELISAs. The results shown are representative of two independent experiments. AU, absorbance units.