Table 2. Reciprocal titres of anti-FMDV antibodies (serotype O) in sera from unvaccinated and rSFV-FMDV vaccinated calves in experiment 2.
| Pre-challenge | Post-challenge | ||||
|---|---|---|---|---|---|
| Group | Vaccination and challenge | Animal | PVD 14 | PVD 28 | PVD 42 |
| 1 | No vaccination (control) | C1 | - | - | 40 |
| Needle challenge | C2 | - | - | 40 | |
| 2 | No vaccination (control) | C3 | - | - | 20 |
| Contact challenge from group 1 | C4 | - | - | 20 | |
| 3 | rSFV-FMDV-P1-2A-mIRES-3C x 2 | C5 | 5 | 20 | 1280 |
| Needle challenge | C6 | 20 | 40 | 2560 | |
| C7 | 40 | 20 | 640 | ||
| 4 | rSFV-FMDV-P1-2A-mIRES-3C x 2 | C8 | - | 5 | 640 |
| Contact challenge from group 3 | C9 | - | 5 | 320 | |
| C10 | 20 | 40 | 1280 | ||
| 5 | rSFV-FMDV-P1-2A-mIRES-3C x 2 | C11 | 40 | 20 | 2560 |
| Contact challenge from group 1 | C12 | 5 | 5 | 2560 | |
| C13 | 5 | 5 | 1280 | ||
Calves C5 to C13 were vaccinated with rSFV-P1-2A-mIRES-3C on PVD 0 and also on PVD 14 and then challenged with FMDV by needle inoculation on PVD 28 (groups 1 and 3) or by contact with inoculated calves from PVD 29 (groups 2, 4 and 5, see text). To determine the antibody titres, sera collected from calves on PVD 14, 28 and 42 were assayed in the ELISA using 2-fold dilutions starting at 1:5.—indicates negative.