Fig 4. YR subtype selectivity of Y₄R PAMs.

Effect of 30 μM compound on the pEC₅₀ of Y-receptor agonists in COS-7 cells stable expressing a Y receptor subtype and the chimeric G-protein G_α6qi4myr. Receptors were stimulated with their native ligands (Y₁R, Y₂R, Y₅R: NPY; Y₄R: PP). For Y-axis values, positive modulation represents an increase in the apparent potency (pEC₅₀) of the native agonist and negative modulation represents a decrease in the apparent potency of the native agonist. Data represent the mean ± SEM of at least two independent experiments (for full concentration-response curves see S2 Fig) (*p < .05, ***p < .001 Bonferroni).