Skip to main content
. 2016 Jun 13;11(6):e0156915. doi: 10.1371/journal.pone.0156915

Fig 4. miR-153-induced anti-glioma cell activity is mediated via downregulating Rictor.

Fig 4

Relative Rictor mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control shRNA (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).