Skip to main content
. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Nature. 2016 Apr 6;533(7601):120–124. doi: 10.1038/nature17657

Figure 2. The predicted mART motif is essential for the role of SdeA in intracellular bacterial growth.

Figure 2

a, The indicated bacterial strains were used to infect D. discoideum and the bacterial yields were monitored at 24-h intervals. Note that SdeA but not the SdeAE/A mutant restored the defect exhibited by the ΔsidEs strain. b, Expression and Dot/Icm-mediated translocation of SdeA and SdeAE/A. The bacteria used for infections were probed for protein expression; the metabolic enzyme isocitrate dehydrogenase (ICDH) was probed as a loading control (upper panels). Saponin-soluble fractions of infected cells were probed for translocated SdeA with tubulin as a loading control (lower panel). c-d, L. pneumophila was used to infect a strain of D. discoideum stably expressing the ER retention fusion GFP-HDEL and the recruitment of the ER marker to the phagosome was evaluated 2 h after infection. IB, immunoblotting. Results in a and c are from one representative experiment done in triplicate from three independent experiments; error bars represent standard error of the mean (s.e.m.) (n=3). Results in b and d are one representative from three independent experiments. Bar, 5 μm. b, Uncropped blots are shown in Supplementary Fig. 1.