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1H-15N TROSY spectra of 150 μM mature PR20D25N dimer in 20 mM sodium phosphate, pH 5.7, recorded at 20 °C in (a) 1, (b) 900 and (c) 1500 bar of pressure, showing the slow exchange process characterizing the pressure-induced unfolding as the cross peaks of the native and those of the unfolded states are both visible at intermediate pressure. (d) A complete recovery of the folded spectrum was observed when the pressure was lowered back to 1 bar after a high-pressure cycle.
