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. Author manuscript; available in PMC: 2017 Jun 13.
Published in final edited form as: Integr Biol (Camb). 2016 Jun 13;8(6):720–728. doi: 10.1039/c6ib00027d

Figure 4. Mechanically primed hepatic stellate cells lose actin stress fiber organization following culture on stiff-to-soft hydrogels.

Figure 4

(A) Representative images and (B) quantification of actin organization for freshly isolated stellate cells cultured for 7 days on soft hydrogels or glass. Blue: nuclei, Green: α-SMA, Red: F-actin. (C) Representative images and (D) quantification of actin organization for stellate cells cultured for 21 days total on soft hydrogels (soft static, dashed lines) or mechanically primed stellate cells cultured for an additional 14 days on soft, stiff, or stiff-to-soft hydrogels. Blue: nuclei, Green: α-SMA, Red: F-actin. (Dashed lines: soft static control). ***: P < 0.001. Scale bars: 100 μm. n > 26 cells per group.