Fig. 3.

Examination of adequate recovery period from anesthesia for saliva sampling in stress experiment using adult male ICR mice tested as individuals. On 1, 3 and 7 days (Day 1, Day 3 and Day 7, respectively) after the prior saliva collections (intact control groups), saliva was collected again following restraint stress (60 min) by the restrainer. Each value represents the mean ± SE of seven to ten animals. The open bars show intact control groups, and the hatched bars show restraint stress groups. Statistically significant corticosterone levels were determined by Student’s paired t-test (*P<0.05, ***P<0.001).