Figure 5.

No impact of IDE deletion on antigenic peptide presentation. MEF were infected with BAC-derived parental virus mCMV-BAC^W, expressing IDEs (green bars), or mutant virus mCMV-Δ4IDE (red bars), lacking IDEs. Infected MEF presenting antigenic peptides derived from the three kinetic phases of viral gene expression, “immediate early” (IE), “early” (E), and “late” (L), were used as stimulator cells in an ELISpot assay. Cells of epitope-specific CTLL were used as responder cells. Bars represent frequencies of CTL responding to the stimulation with secretion of IFN-γ.