Table 1.
Floral nectar proteins of Liriodendron tulipifera Linn. (South Carolina accession) annotated by LC-MS/MS after 2-D gel electrophoresis.
| Spot | Accession | Protein name | Score | Coverage % | MW/pI∗ | Hit Peptides | Species |
|---|---|---|---|---|---|---|---|
| 1 | A2Y7R5 | GTP-binding nuclear protein Ran-2 | 225 | 19 | 25.36/6.66 | LVIVGDGGTGK HLTGEFEK VCENIPIVLCGNK NLQYYEISAK |
Oryza sativa Indica Group |
| 2 | P15252 | Rubber elongation factor protein | 160 | 34 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis |
| 3 | A2Y7R5 | GTP-binding nuclear protein Ran-2 | 162 | 21 | 25.36/6.66 | HLTGEFEK LVIVGDGGTGK NLQYYEISAK VCENIPIVLCGNK |
Oryza sativa Indica Group |
| 4 | P15252 | Rubber elongation factor protein | 244 | 27 | 14.71/5.04 | SGPLQPGVDIIEGPVK DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis |
| 5 | Q43209 | Protein-L-isoaspartate O-methyltransferase | 451 | 7 | 24.81/4.90 | VAEVMETIDR YVPLTSR |
Triticum aestivum |
| 6 | P11143 | Heat shock 70 kDa protein | 814 | 18 | 70.87/5.22 | VEIIANDQGNR TTPSYVAFTDTER QFAAEEISSMVLIK EIAEAYLGSTIK NAVVTVPAYFNDSQR DAGVIAGLNVMR IINEPTAAAIAYGLDK ATAGDTHLGGEDFDNR MVNHFVQEFK |
Zea mays |
| 7 | O82089 | Copper transport protein CCH | 255 | 12 | 13.08/4.91 | MEGVESFDIDIK MEGVESFDIDIKEQK |
Arabidopsis thaliana |
| 10 | Q9FRL8 | Glutathione S-transferase DHAR2 | 115 | 9 | 23.51/5.79 | VLLTLEEK NWSVPESLTSVR |
Arabidopsis thaliana |
| 11 | Q0JNR2 | Cysteine proteinase inhibitor 12 | 322 | 3 | 27.25/6.07 | ENALLEFVR | Oryza sativa Japonica Group |
| 12 | Q9SVD7 | Ubiquitin-conjugating enzyme E2 variant 1D | 264 | 34 | 16.69/6.20 | TLGSGGSSVVVPR LLEELER GIGDGTVSYGMDDGDDIYMR KLVQPPEGTCF |
Arabidopsis thaliana |
| 13 | P49310 | Glycine-rich RNA-binding protein GRP1A | 634 | 38 | 16.06/5.21 | ASPDVEYR CFVGGLAWATDDR DAIEGMNGQDLDGR SITVNEAQSR SGGGGGYGGGGGGYGGGGR |
Sinapis alba |
| 14 | A2XKU9 | Costars family protein | 173 | 27 | 9.63/5.78 | VTFGVIFNDDR CANIFEALVGTLR |
Oryza sativa Indica Group |
| 15 | P29449 | Thioredoxin H-type 1 | 2221 | 18 | 14.12/5.62 | KLVVVDFTASWCGPCR LVVVDFTASWCGPCR VDVDELK |
Nicotiana tabacum |
| 16 | P15252 | Rubber elongation factor protein | 378 | 52 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR FSYIPNGALK FVDSTVVASVTIIDR DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis |
| P29449 | Thioredoxin H-type 1 | 217 | 18 | 14.12/5.62 | VDVDELK LVVVDFTASWCGPCR KLVVVDFTASWCGPCR |
Nicotiana tabacum | |
| 17 | Q84JT6 | Peptide methionine sulfoxide reductase B9 | 128 | 12 | 15.75/6.81 | AILSPEQFR HCVNSVSLK |
Arabidopsis thaliana |
| 18 | P35135 | Ubiquitin-conjugating enzyme E2-17 kDa | 237 | 47 | 16.68/7.71 | ELKDLQK VFHPNINSNGSICLDILK EQWSPALTISK VLLSICSLLTDPNPDDPLVPEIAHMYK AKYETTAR |
Solanum lycopersicum |
| Q03250 | Glycine-rich RNA-binding protein 7 | 633 | 21 | 16.94/5.85 | SITVNEAQSR CFVGGLAWATDDR DAIEGMNGQDLDGR |
Arabidopsis thaliana | |
| Q9FVI1 | Actin-depolymerizing factor 2 | 168 | 13 | 16.67/5.78 | MIYASSK IFFIAWSPDTAR |
Petunia × hybrida | |
| 19 | Q94A97 | Ubiquitin-conjugating enzyme E2 35 | 223 | 27 | 17.24/6.74 | LLSEPAPGISASPSEDNMR LELFLPEEYPMAAPK IYHPNIDK |
Arabidopsis thaliana |
| P55142 | Glutaredoxin-C6 | 61 | 30 | 11.94/5.77 | TVPNVFINGK AIELDGESDGSELQSALAEWTGQR |
Oryza sativa Japonica Group | |
| 20 | P69310 | Ubiquitin | 281 | 61 | 8.52/6.56 | TITLEVESSDTIDNVK IQDKEGIPPDQQR EGIPPDQQR TLADYNIQK ESTLHLVLR |
Avena sativa |
| 21 | P0CH10 | Ubiquitin-60S ribosomal protein L40 | 2014 | 54 | 14.92/9.94 | EGIPPDQQR TLADYNIQK ESTLHLVLR MQIFVKTLTGK LIFAGKQLEDGR IQDKEGIPPDQQR TITLEVESSDTIENVK TLADYNIQKESTLHLVLR |
Chlamydomonas reinhardtii |
| 22 | P84718 | Putative oxygen-evolving enhancer protein 1 | 141 | 24 | 12.27/4.35 | VINTWADIINR GGSTGYDNAVALPAGGR |
Pinus strobus |
| 23 | P69310 | Ubiquitin | 184 | 61 | 8.52/6.56 | TITLEVESSDTIDNVK IQDKEGIPPDQQR TLADYNIQK ESTLHLVLR |
Avena sativa |
| P15252 | Rubber elongation factor protein | 318 | 44 | 14.71/5.04 | NVAVPLYNR SLASSLPGQTK FSYIPNGALK SGPLQPGVDIIEGPVK FVDSTVVASVTIIDR |
Hevea brasiliensis | |
| 24 | P29449 | Thioredoxin H-type 1 | 285 | 18 | 14.12/5.62 | KLVVVDFTASWCGPCR LVVVDFTASWCGPCR VDVDELK |
Nicotiana tabacum |
| 25 | P0C030 | Ubiquitin-NEDD8-like protein RUB1 | 1629 | 62 | 17.12/5.77 | TITLEVESSDTIDNVK IQDKEGIPPDQQR EGIPPDQQR TLTGKEIEIDIEPTDTIDR EIEIDIEPTDTIDR VEEKEGIPPVQQR EGIPPVQQR DYNIEGGSVLHLVLALR |
Oryza sativa Japonica Group |
| A2XKU9 | Costars family protein | 181 | 27 | 9.63/5.78 | VTFGVIFNDDR CANIFEALVGTLR |
Oryza sativa Indica Group | |
| 26 | P29449 | Thioredoxin H-type 1 | 1314 | 25 | 14.12/5.62 | VDVDELK EVDRVVGAK LVVVDFTASWCGPCR KLVVVDFTASWCGPCR |
Nicotiana tabacum |
| Q03250 | Glycine-rich RNA-binding protein 7 | 675 | 21 | 16.94/5.85 | CFVGGLAWATDDR DAIEGMNGQDLDGR SITVNEAQSR |
Arabidopsis thaliana | |
| B4YYA9 | Costars family protein ST45-2 | 291 | 26 | 10.19/5.63 | VTFGVLFNDDR CANIFEALVGTLR |
Eutrema halophilum | |
| 27 | P0C030 | Ubiquitin-NEDD8-like protein RUB1 | 265 | 42 | 17.12/5.77 | TITLEVESSDTIDNVK TLADYNIQK EIEIDIEPTDTIDR EGIPPVQQR DYNIEGGSVLHLVLALR |
Oryza sativa Japonica Group |
| 28 | P15252 | Rubber elongation factor protein | 215 | 41 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR FSYIPNGALK DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis |
| 29 | P15252 | Rubber elongation factor protein | 530 | 52 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR FSYIPNGALK FVDSTVVASVTIIDR DASIQVVSAIR |
Hevea brasiliensis |
| 30 | P15252 | Rubber elongation factor protein | 283 | 41 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR FSYIPNGALK DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis |
| 31 | A4KA43 | Profilin-6 | 120 | 9 | 14.19/4.90 | YMVIQGEPGVVIR | Corylus avellana |
| P15252 | Rubber elongation factor protein | 111 | 44 | 14.71/5.04 | NVAVPLYNR SLASSLPGQTK DASIQVVSAIR SGPLQPGVDIIEGPVK FVDSTVVASVTIIDR |
Hevea brasiliensis | |
| 32 | A2YIW7 | Thioredoxin H-type | 112 | 5 | 13.32/5.16 | VDVDELK | Oryza sativa Indica Group |
| 33 | Q9C996 | GDSL esterase | 109 | 2 | 40.65/8.38 | CFGKMNVMAK | Arabidopsis thaliana |
| 34 | P0DH9 | ADP-ribosylation factor 2-B | 124 | 19 | 20.64/6.43 | ILMVGLDAAGK DAVLLVFANK QDLPNAMNAAEITDK |
Glycine soja |
| 35 | O49886 | Peptidyl-prolyl cis-trans isomerase | 98 | 12 | 18.51/8.36 | TAENFR FADENFIK FADENFIKK TEWLDGK |
Lupinus luteus |
| P49310 | Glycine-rich RNA-binding protein GRP1A | 204 | 33 | 16.06/5.21 | SITVNEAQSR CFVGGLAWATDDR DAIEGMNGQDLDGR |
Sinapis alba | |
| 36 | Q9FVI1 | Actin-depolymerizing factor 2 | 2168 | 13 | 16.67/5.78 | MIYASSK IFFIAWSPDTAR |
Petunia × hybrida |
| 37 | P0CG86 | Ubiquitin-40S ribosomal protein S27a | 158 | 28 | 17.87/9.83 | MQIFVK IQDKEGIPPDQQR TLADYNIQK ESTLHLVLR VDDATGKVTR |
Hordeum vulgare |
| 38 | P15252 | Rubber elongation factor protein | 439 | 52 | 14.71/5.04 | NVAVPLYNR SLASSLPGQTK FSYIPNGALK DASIQVVSAIR FVDSTVVASVTIIDR |
Hevea brasiliensis |
| Q9FVI1 | Actin-depolymerizing factor 2 | 765 | 18 | 16.67/5.78 | MIYASSK QKEVVVEK IFFIAWSPDTAR |
Petunia × hybrida | |
| 39 | Q03250 | Glycine-rich RNA-binding protein 7 | 326 | 21 | 16.94/5.85 | SITVNEAQSR CFVGGLAWATDDR DAIEGMNGQDLDGR |
Arabidopsis thaliana |
| 132270 | Rubber elongation factor protein | 338 | 52 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR FSYIPNGALK FVDSTVVASVTIIDR DASIQVVSAIR SLASSLPGQTK |
Hevea brasiliensis | |
| 40 | Q9FLP6 | Small ubiquitin-related modifier 2 | 439 | 35 | 11.76/5.35 | LMNAYCDR GQDGNEVFFR KLMNAYCDR VKGQDGNEVFFR QSVDFNSIAFLFDGR |
Arabidopsis thaliana |
| Q9FVI1 | Actin-depolymerizing factor 2 | 188 | 13 | 16.67/5.78 | MIYASSK IFFIAWSPDTAR |
Petunia × hybrida | |
| 41 | Q9SNW5 | Profilin-3 | 600 | 16 | 14.27/4.73 | KGSGGVTIK YMVIQGEPGAVIR |
Lilium longiflorum |
| 42 | A4KA43 | Profilin-6 | 169 | 9 | 14.19/4.90 | YMVIQGEPGVVIR | Corylus avellana |
| P15252 | Rubber elongation factor protein | 167 | 26 | 14.71/5.04 | SGPLQPGVDIIEGPVK NVAVPLYNR SLASSLPGQTK |
Hevea brasiliensis | |
| 43 | Q9ZSW9 | Translationally-controlled tumor protein homolog | 673 | 27 | 19.08/4.50 | QFVTYMK VVDIVDTFR LQEQPAFDK MLVYQDLLTGDELLSDSFPYK |
Hevea brasiliensis |
| Q0JNS6 | Calmodulin-1 | 181 | 37 | 16.88/4.11 | ELGTVMR HVMTNLGEK LTDEEVDEMIR DTDSEEELKEAFR VFDKDQNGFISAAELR |
Oryza sativa Japonica Group | |
| 44 | Q5Z9Z3 | Thioredoxin-like protein Clot | 219 | 7 | 15.34/4.88 | LTGVPTLIR FRLTGVPTLIR |
Oryza sativa Japonica Group |
∗The numerical value of Mw and pI just represent the accession, not the experimental value.