FIGURE 4.
Expression of methyltransferase and DNA demethylase genes in normal and mutant tomato fruits. Heat maps from normalized microarray data (see Liu et al., 2015, Supplementary information) showing expression of methyltransferase and DNA demethylase genes in developing and ripening tomato fruits from Ailsa Craig and near isogenic lines containing the non-ripening (nor), ripening inhibitor (rin), and Colourless non-ripening (Cnr) mutations. Fruits were sampled at 15, 20, 25, 30, and 40 days post anthesis (dpa) and also at breaker (Br) and for 7 days post breaker (BR+1 to BR+7). Forty dpa was taken as mature green and BR+7 was the red ripe stage. The values used to construct the maps were means of three biological replicates (individual fruits) at each stage at for each gene. SlMET1 (Solanum lycopersicon, CYTOSINE-DNA-METHYLTRANSFERASE 1), DML (DNA DEMETHYLASE), DRM (DOMAINS REARRANGED METHYLASE). On the heat map red is for high levels of gene expression and green for low expression. Yellow represents intermediate values.