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Allergic asthma and aerobic exercise experimental model. Male Balb/c mice (n = 8/group) received OVA i.p. injections (20 μg) on day 0, 14, 28, and 42. The 30-min aerosol treatments for OVA (1%) were given 3 days a week starting from day 21. Aerobic exercise training was performed 30 min a day, 5 days a week starting from day 28 and ending on day 52. Euthanasia and/or metacholine challenge (25 and 50 mg/mL) was performed on day 54.
