Table 1.
Comparison of the three protein-extraction methods using maize leaf midribs.
| Method | Protein yield (mg/g dry weight) | Spot number Aa | Time (h)b | Match to all (correlation of coefficient %) | Match rate (%)c | Spot in red boxd |
|---|---|---|---|---|---|---|
| Phenol extraction | 1.64 ± 0.01 | 655 ± 9 | 1.5 | 285 (0.843) | Master gel | 239 ± 13 |
| TCA/acetone extraction | 3.08 ± 0.21 | 589 ± 25 | 1.0 | 208 (0.830) | 62 ± 6 | 109 ± 18 |
| TCA/acetone/phenol extraction | 1.77 ± 0.04 | 545 ± 6 | 2.5 | 215 (0.831) | 53 ± 3 | 133 ± 11 |
a
Spot number in 2DE images detected using auto spot detection module of PDQUEST software (Bio-Rad).
b
Time consumed by each protein extraction method.
c
Match rate calculated taking gel image of phenol extraction as the master.
d
Spot numbers in red boxes of Figure 3.