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. 2016 Jun 14;7:861. doi: 10.3389/fpls.2016.00861

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Copyright © 2016 Li, Li, Gou, Wang, Fan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LC-MS analysis of the products from the in vivo assays in yeast cells. (A)–(F) show the products of the PlOMT9-expressed cells fed with the substrates genistein yielding biochanin A (peak 1) and 4′,7-dimethoxy-5-hydroxyisoflavone (peak 2), daidzein yielding formononetin (peak 3) and 4′,7-dimethoxyisoflavone (peak 4), prunetin yielding 4′,7-dimethoxy-5-hydroxyisoflavone (peak 5), isoformononetin yielding 4′,7-dimethoxyisoflavone (peak 6), formononetin yielding 4′,7-dimethoxyisoflavone (peak 7), and biochanin A yielding 4′,7-dimethoxy-5-hydroxyisoflavone (peak 8), respectively. WAT11 (pESC-HIS) represents the strain carrying the empty vector pESC-HIS as a control; WAT11 (pESC-HIS-PlOMT9) represents the strain expressing PlOMT9. The mass spectra of the peaks 1–8 and their corresponding authentic standards are shown in Supplementary Figure S1.