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. 2016 Jun 14;7:861. doi: 10.3389/fpls.2016.00861

FIGURE 5.

FIGURE 5

Measurement of methylated isoflavone accumulation and gene expression in P. lobata. (A) The concentration of formononetin in P. lobata organs analyzed by HPLC; (B) and (C), the relative transcript abundance of the PlOMTs in P. lobata organs measured by qRT-PCRs; (D), the effect of the MeJA treatment on the biosynthesis of formononetin in the P. lobata roots; (E), the regulation of the MeJA elicitation on the gene expression of PlOMT9 in the roots. The P. lobata actin gene (GenBank accession number HO708075) was used as an internal standard in the qRT-PCRs. Each measurement was performed in three biological replicates. Asterisks indicate significant differences, P < 0.05.