FIGURE 6.

The concentrations of formononetin, ononin, daidzein, and daidzin in transgenic soybean hairy roots. PlOMT-GFP, the hairy roots transformed with pCAMBIA1302-PlOMT9; free GFP, the hairy roots transformed with the empty vector pCAMBIA1302; MeJA treatment, the hairy root cultures were treated with MeJA at the final concentration of 100 μM; Mock control, the hairy root cultures were treated with the same concentration of 0.001% ethanol. After 10 days of the treatments, the hairy roots were collected for measuring the metabolites by HPLC analysis. Each measurement was performed in three biological replicates.