Table 1.
Strains and vectors used in this study
| Strains or plasmids | Features | References |
|---|---|---|
| Pseudomonas aeruginosa | ||
| PAO1 | Wild‐type P. aeruginosa | (Stover et al. 2000) |
| CF_PA1 to CF_PA125a | CF clinical P. aeruginosa strains | (Dingemans et al. 2014) |
| Escherichia coli | ||
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA‐argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi‐1 gyrA96 relA1 | (Hanahan 1985) |
| BL21(DE3)pLysS | E. coli B F– dcm ompT hsdS(rB – mB –) gal λ(DE3) [pLysS Camr] | (Weiner et al. 1994) |
| Plasmids | ||
| pET15b | Expression vector, N‐terminal his‐tag, Apr | (Studier et al. 1990) |
| pJB3Tc20 | Broad‐host‐range shuttle vector, Apr and Tcr | (Blatny et al. 1997) |
a
With the exception of CF_PA8, CF_PA18, CF_PA21, CF_PA25, CF_PA29 to CF_PA33, CF_PA52 and CF_PA95 to CF_PA99. Camr and Apr indicate resistance to chloramphenicol and ampicillin, respectively