Figure 3.

Cross‐complementation assay of Vibrio FlhA in the absence of FliH and FliI. (A) Motility of NH004 transformed with pNY101 (∆fliH‐fliI flhB* VaflhA) in soft agar. MMHI0117 carrying pSU41 (∆fliH‐fliI flhB*) and NH004 harboring pSU41 (∆fliH‐fliI flhB* ∆flhA) were used as the positive and negative controls. Plates were incubated at 30°C for 16 h. (B) Immunoblotting, using polyclonal anti‐FlgD antibody, of whole‐cell proteins (Cell) and culture supernatant fractions (Sup) prepared from the above strains.