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. 2016 Feb 25;5(3):424–435. doi: 10.1002/mbo3.340

Figure 4.

Figure 4

Isolation of pseudorevertants from the ∆ fliH‐fliI flhB(P28T) VaflhA strain. (A) Motility of NH004 transformed with pNY101 (∆fliHI flhB* VaflhA) and its pseudorevertants, MMA2002 (∆fliH‐fliI flhB* VaflhA flgM(P8S)), MMA2003 (∆fliH‐fliI flhB* VaflhA flgM(P11L)), and MMA2004 (∆fliH‐fliI flhB* VaflhA flgM 45f‐s) at 30°C for 8 h. (B) Immunoblotting, using polyclonal anti‐FlgD antibody, of whole‐cell proteins (Cell) and culture supernatant fractions (Sup) prepared from the above strains. (C) Location of extragenic flgM suppressor mutations. FlgM consists of 97 amino acid resides. The sites of suppressor mutations in FlgM are shown by arrows. Missence mutations are indicated as P8S and P11L. A frameshift mutation is indicated by f‐s. The N and C termini of FlgM are labeled as 1 and 97, respectively.