Figure 5.

C. elegans can be used to measure virulence of M. marinum. (A–D) Relative survival of C. elegans infected with M. marinum wild type (wt) and macrophage infection mutants (MIMs), 2 d postinfection (day 6). Three trials of 20 nematodes (n = 60) were infected with each of the MIM strains and survival was assessed on day 6. All survival calculations are relative to survival after infection with wild type, e.g. relative survival = number of nematodes surviving with strain/number of nematodes surviving with wild type. Data are presented for means and standard deviations. P values are provided comparing relative survival with each MIM to infection with wild type (unpaired t‐test).