Figure 5.

The higA mutant has less swarming compared to the wild‐type strain. Pseudomonas aeruginosa PA14, the higA mutant, and the negative control (rhlR mutant) were grown overnight in LB medium. The culture (1 μL) was inoculated into the middle of a BM2 plate (Overhage et al. 2008) that was dried for 3 h before inoculation, and the plates were incubated for 18 h. Swarming plates of (A) P. aeruginosa PA14 with 52 ± 8% area coverage, (B) the higA mutant with 11 ± 2% area coverage, and (C) the rlhR mutant (negative control) with 5 ± 0.4% area coverage. Results shown are from one of three representative independent cultures.