Skip to main content
. 2016 Jun 14;7:234. doi: 10.3389/fphys.2016.00234

Table 1.

DNA sequence of primers used to detect gene expression by semi-quantitative RT-PCR.

GENE FORWARD (5′-3′) REVERSE (5′-3′)
Cu transporter Ctr1 GGGCTTGGTAGAAGTCCGTA GAAAGTATCCCGTCCCAGCC
Ctr2 CCGCAATCCTAGTCGAGTCC GTGGTCTGTCCCCTAAAGGC
Atp7a GTGGGCTGGGAAAGCCG GTGCTGTGCTCTTCACAAGC
Atp7b GATGAAAGGACAGACGGCCA TGCACTGCTCTTCATCCCTG
Cu chaperon Ccs GAACCATCGACGGCCTAGAG GCTACAGCACTTATCTGCCCT
Atox1 ACTGCCCGTGTGTGCC GCCAAGGTAGGAAACAGCCT
Cox17 CAGGGTAGTCGGAGTTTGGG TCACAAAGTAGGCCACCACG
Cu protein Sod1 AACCATCCACTTCGAGCA CAATCCCAATCACTCCAC
Aoc3 TGGGTTTTACCCTCACCCCAT TCCGGTTGCCAAGGTACAAT
Lox AGGGCGGATGTCAGAGACTA AATCCCTGTGTGTGTGCAGT
Other genes Gapdh CCAATGTGTCCGTCGTGGATCT GTTGAAGTCGCAGGAGACAACC
Tnf-α TAGCCCACGTCGTAGCA GGGGTCAGAGTAAAGGGGTC
Vegf ACTGGACCCTGGCTTTACTG CTTGCGCTTTCGTTTTTGACC
Fak AGCTTCAGCCCCAGGAAATC TGCTGATGAGCTCGCCTAAG