Figure 3.

(A) Must fermentation performed by simultaneous inoculum of beads containing S. cerevisiae and O. oeni cells separately immobilized, (sample C2). (B) Must fermentation performed by simultaneous inoculum of beads containing S. cerevisiae and an inoculum of free (10⁶ CFU/ml) O. oeni cells (sample C3). (C) Fermentation using beads containing S. cerevisiae cells, followed, at the end of AF, by beads containing O. oeni (sample C4). (D) Fermentation performed by using simultaneous inoculum of (10⁶ CFU/ml) free S. cerevisiae and (10⁶ CFU/ml) O. oeni cells (sample C5); CO2 (g/l) production , Malic acid consumption. Inoculation time of S. cerevisiae , inoculation time of O. oeni.