Fig. 6.

Kinases phosphorylation in response to NAC treatment. (A) pJNK expression was evaluated; the results were normalized to JNK expression. (B) pERK½ expression was evaluated; the results were normalized to ERK½ expression. Comparison of control cells (CC); MDI cells (DC) and MDI + NAC treated cells (DCN-0.01: 0.01 mM NAC; DCN-0.05: 0.05 mM NAC; DCN-1: 1 mM NAC; DCN-5: 5 mM NAC). The results are expressed as arbitrary units, arbitrary units of control cells (CC) was set to 1. The values represent the fold increase in protein expression compared to control cells (CC). The bars show the average of two different experiments (means±SD). Representative results from one of two independent western blot experiments with similar results are shown.