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Examples of currently available PCR methods using RUO reagents.
| Features | CastPCR (ThermoFisher) | PCR Clamping | High Resolution Melt |
|---|---|---|---|
| Starting material | DNA | DNA | DNA |
| Coverage | Depends on plate layout | Depends on primers chosen |
Depends on primers chosen |
| Analytical Sensitivity (LoD) | <5% | <5% | <5% |
| Neoplastic cell% limit | 10% | 10% | 10% |
| Duration of test | 3 h | 3–4 h | 3–4 h |
| Reagents | Lyophilised in plate + master mix (liquid) | Liquid | Liquid |
| Results | Manual | Manual | Manual |
