Table 1.

UPLC gradient program. Eluent A: 5.0 mM ammonium acetate, pH 5. Eluent B: acetonitrile.

Time (min.)	Flow (µL/min)	%A	%B
0.0	200	100	0
0.5	200	100	0
12.0	200	95	5
14.6	200	10	90
15.0	300	10	90
16.0	300	10	90
17.0	200	100	0
20.0	200	100	0