Table 3.
The certified values, ratios of HER2 copies to the reference genes, 95% uncertainty interval and 68% prediction interval.
| Component | Cell line | Ratio | 95% uncertainty interval | 68% prediction interval |
|---|---|---|---|---|
| A | SK-BR-3 | 9.7 | 8.7–10.7 | 5.8–20.8 |
| B | MDA-MB-231 | 1.3 | 1.1–1.5 | 0.6–1.9 |
| C | MDA-MB-361 | 6.4 | 5.7–7.1 | 4.8–14.2 |
| D | MDA-MB-453 | 2.9 | 2.6–3.2 | 1.4–7.2 |
| E | BT-474 | 17.7 | 15.9–19.5 | 11.7–45.3 |
The uncertainty intervals characterize the uncertainty in the long-run averages of many repeated measurements. The 68% prediction interval is used for single measurements. The distribution of individual observations is expected to exhibit substantially greater variability, as evidenced by the much broader and lower coverage (68% vs 95%) prediction intervals.