Fig. 1.

Metabolic measurements of tumor cells under normal culture conditions. (a) TPEF of MDA-MB-231 cells, NADH channel; (b) TPEF of MDA-MB-231 cells, ${\mathrm{FAD}}^{+}$ channel. The outlined ROI selects cellular cytoplasm for calculation of the ORR. (c) n-OCR and the ORR for MCF-7 and MDA-MB-231 cells. The measurements from each cell line are compared with two-tailed ANOVA. Both measurements demonstrate statistically different metabolic rates for MCF-7 cells and MDA-MB-231 cells ($p<0.05$). The error bars for n-OCR come from the standard deviation of 12 different wells ($50,000\text{\hspace{0.17em}\hspace{0.17em}cells}/\text{well}$), and the error bars for ORR come from measurements on 25 different cells. The scale bar indicates $10\mu \mathrm{m}$.