Fig. 2.

Dynamic monitoring of MCF-7 cell metabolism by TPEF microscopy (circles, dashed line) and seahorse flux analyzer (diamonds, solid line). Cells in regular culture conditions were measured at time $T=0$ and then treated with oligomycin, FCCP, and rotenone/antimycin A at $T=\mathrm{0,15}$, and 30 min in sequence. The n-OCR and ORR were measured every 5 min. The error bars for n-OCR come from the standard deviation of 12 measurements, and the error bars for ORR come from measurements on 25 different cells.