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. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656

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(a) Split-ubiquitin assays using STLs as bait and CesAs as prey in yeast. Values are the percentage of yeast colonies that displayed growth after 4 days on selective medium at 28 °C. ALG5 (yeast ALG5 dolicholphosphoglucose synthetase) was fused with wild-type N-terminal part of ubiquitin (NubI) as positive control. Mutated N-terminal part of ubiquitin (NubG) was used in the rest of the vectors including the auto-activation control (empty vector), negative control (ALG5 and Got1p) and CesAs. (b) BiFC assays detecting the interaction of CesAs and STLs in N. benthamiana epidermal leaf cells. The N-terminal (Yn) or C-terminal (Yc) part of VENUS was fused in frame with CesA1, CesA3, CesA6, STL1, STL2 and Got1p (negative control), respectively. The combination of constructs is indicated in each figure panel. The nuclear marker CFP-N7 (cyan) was included as a positive transformation control in all experiments. Scale bar, 50 μm.