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. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656

Figure 8. STL function is necessary for CSC dynamics and assembly.

Figure 8

(a) Photo-bleaching of three-day-old wild-type or stl1stl2 hypocotyl cells expressing GFP-CesA3. Scale bar, 5 μm. (b) Box plot displaying re-population of the plasma membrane with fluorescent CesA foci (given as mean delivery rates of CesAs per area unit per hour). n≥11 cells from more than 6 seedlings per genotype. ***P value<0.001, Student's t-test. (c) Time average images of GFP-CesA3 at the plasma membrane focal plane of 3-day-old etiolated Col-0 and stl1stl2 mutant cells. Scale bar, 10 μm. (d) Box plot of GFP-CesA3 speed estimates from cells such as those in c. n≥10 cells from 4 seedlings per genotype. ***P value<0.001, Student's t-test. In both b and d, centre lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to minimum and maximum values. (e–h) Protein amount of CesA and CSC in wild-type and stl1stl2 mutants. Fourteen-day-old wild-type and stl1stl2 mutants expressing GFP-CesA3 (e,f) or stems of 5-week-old wild-type and stl1stl2 mutants (g,h) were used for microsome preparation (e,g) or total protein extraction (f,h). The samples were analysed by BN-PAGE (e,g) or SDS-PAGE (f,h) followed by western blot with anti-GFP (e,f) or anti-CesA8 antibody (g,h). Ponceau S staining of the membranes, coomassie blue (CBB) staining of protein gels, western blot with anti-actin or unspecific bands are shown for protein loading control. (i) Relative band intensity of western blot in stl1-1stl2-2 samples (normalized with the band intensity in control). Values are mean (±s.e., n=3). **P value<0.01 and *P value<0.05, Student's t-test.