Table 2.

SAXS Data Collection and Derived Parameters for DAPK2 and Mutants

	WT	D220K	BL
Data Collection Parameters
Instrument	EMBL P12 (PETRA-III)
Beam geometry (mm2)	0.2 × 0.12
Wavelength (Å)	1.24
s range (Å−1)a	0.003–0.45
Exposure time (s)	0.045·20
Sample concentration (mg/ml)	2.0–15.0	2.0–15.0	2.0–15.0
Temperature (K)	283
Structural Parameters
I(0) (cm−1) (from p(r))	0.027 ± 0.001	0.025 ± 0.001	0.021 ± 0.001
Rg (Å) (from p(r))	26.4 ± 0.2	25.9 ± 0.2	23.4 ± 0.2
Dmax (Å)	81 ± 5	90 ± 5	75 ± 5
Dry volume (103 Å3) calculatedb	45
Contrast (Δρ × 1010 cm−2)	2.704
Molecular mass Mr (kDa) (from I(0))	41 ± 5	37 ± 4	32 ± 3
Molecular mass Mr (kDa) (from Porod volume [Vp/1.7])	59 ± 6	52 ± 5	46 ± 5
Calculated monomeric mass Mr (kDa)b	37.3
Software Employed
Primary data reduction	Automated pipeline (Blanchet et al., 2015)
Data processing	ALMERGE, AUTORG, DATGNOM, DATPOROD
Computation of model intensities	CRYSOL, FFMAKER, OLIGOMER

^aMomentum transfer |s| = 4πsin(θ)/λ.

^bCalculated from the sequence using www.basic.northwestern.edu/biotools/proteincalc.html.