Figure 6.

KAT7 and RSF1 Tetherings Reduce H3K9me3 Modification

(A) Scheme of candidate protein tethering and H3K9me3 immunostaining in G₁ phase.

(B) Examples of H3K9me3 staining. Fluorescence images were obtained with DAPI (DNA), EYFP (green), and anti-H3K9me3 antibody (red). Arrowheads indicate loci of tetR-EYFP-fusion protein spots on the alphoid^tetO DNA integration site. Scale bars, 5 μm.

(C and D) Observed H3K9me3 reduction frequency was plotted (>50 tetR-EYFP spots were counted in each sample). Data are presented as mean ± SD (n = 3).

(E) Scheme of micronuclei formation combined with siRNA transfection (left). Cells were stained and observed as shown in Figure 5D. The frequencies of the CENP-B-positive and -negative micronuclei were plotted (>200 cells were counted in each cell line). Data are presented as mean ± SD (n = 3). ^∗p < 0.05, ^∗∗p < 0.005 (t test).