Figure 7.

KAT7 and RSF1 Promote Histone Exchange/Turnover

(A) A model for histone turnover. Note that KAT7 acetylates not only histone H3 but also H4 (Miotto and Struhl, 2010).

(B) Scheme for experiments.

(C and D) Fluorescence images were obtained with DAPI (DNA), EYFP (green), and Halo-TMR ligand (red). The Halo-histone H3.3 (C) or -CENP-A (D) assembly frequency was plotted (>50 tetR-EYFP spots were counted in each sample). Data are presented as mean ± SD (n = 3). Arrowheads indicate loci of tetR-EYFP-fusion protein spots on the alphoid^tetO DNA integration site. Scale bars, 5 μm.