Figure 2.

Correlation between Requirements for ER MCSs with EGFR-MVBs and ILV Formation

(A) HeLa cells were stained for endogenous S100A11 using pre-embedding labeling. Staining (white arrows) is visible on the ER (arrowheads) and at MCSs (black arrows) between the ER and MVBs. Scale bar, 200 nm.

(B and C) HeLa cells transfected with non-targeting siRNA (CON) and siRNA targeting S100A11 (siS100) or incubated with BAPTA were stimulated with EGF and anti-EGFR gold and MCSs with EGFR-MVBs (B) and ILVs/EGFR-MVB (C) quantified. Data are means ± SD of three experiments. ^∗∗p < 0.01.

(D–F) HeLa cells mock transfected (Control) or transfected with WT annexin A1 or annexin A1 mutants were stimulated and quantified as above. Data are means ± SD of three experiments. ns, not significant p > 0.05; ^∗p < 0.05, ^∗∗p < 0.01.

See also Figure S2.