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. 2016 Jun 7;110(11):2551–2561. doi: 10.1016/j.bpj.2016.05.002

Figure 1.

Figure 1

Stimulus and analysis paradigms. (A) Traces of voltage protocol. Step voltages (−160 mV to +100 mV by 20 mV increments) were delivered for 700 ms followed by a return to a holding potential of 0 mV for 40 ms. Superimposed on the steps were summed discrete dual-sine frequencies 655 ms in duration (see Materials and Methods). (B) Elicited currents (offset for easy visualization), AC and step-induced, were used to extract capacitance and integrated charge movements (dashed ovals), respectively. (C) Averaged NLC traces of OHCs with intracellular chloride clamped to 1 or 140 mM chloride. NLC was estimated from the latter half of the dual-sine stimulation duration. (D and E) Corresponding displacement currents extracted by linear capacitive current subtraction (D) and extracted Q-V curves (E) at 1 mM and 140 mM chloride conditions for an equivalent interrogation time based on fits of NLC with Eq. 1 (blue traces) or on fits of integrated off charge with Eq. 2 (red traces) give comparable results, as expected (see Materials and Methods for details). To see this figure in color, go online.