Figure 2.

Measured values of T_mix and Δh for 48:32:20 DiPhyPC/DPPC/chol bilayers. (A) Fluorescence micrographs for a single GUV (top) and a graph of percent demixed GUVs versus temperature for a population of GUVs (bottom). The solid blue line is a sigmoidal fit of the black points. Dashed lines bound the 95% confidence interval. The star lies at T_mix, and the bar spans the uncertainty; here T_mix = 40.7 ± 0.3°C. (B) AFM scan of a supported lipid bilayer at 22°C (top) and height histogram of the same scan (bottom). The blue line follows two Gaussian peak fits of the black data points. For a single scan, Δh is reported as the distance between the mean thicknesses of the two peaks, and uncertainty is reported as the standard deviation of the bootstrap fit, here Δh = 1.39 ± 0.02 nm. Values of T_mix and Δh are listed in Table S2 in the Supporting Material. To see this figure in color, go online.