Figure 2. Wing to haltere transformations in Drosophila caused by the ectopic-expression of Ubx_Apis.

(A–C) vg-GAL4/UAS-Ubx_Apis adult fly with reduced and deformed wing. The separated wing is shown at higher magnifications (B,C). Note trichomes are shorter and more densely arranged. (E,F) Wildtype larval wing (E) and haltere (F) discs stained for Wg (green). While Wg is expressed in in both anterior and posterior compartments in the wing disc, it is not expressed in the posterior compartment (arrow in F) of the haltere disc. (G) vg-GAL4/UAS-Ubx_Apis larval wing disc stained for Wg (green) and Ubx_Apis (red). Note repression of Wg by Ubx_Apis is only in the posterior compartment (arrow in G’), an indication of homeotic transformation. (H,I) vg quadrant-lacZ larval wing (H) and haltere (I) discs stained for lacZ (green). (J) vg-GAL4/UAS-Ubx_Apis; vg quadrant-lacZ larval wing disc stained for lacZ (green) and Wg (red). Note, non cell-autonomous repression of vg quadrant-lacZ. Repression of Wg in the posterior compartment is more pronounced in J (arrows) than in G. In all imaginal discs, posterior is to the right and ventral to the top.