Figure 7. The putative quadrant enhancer of vg of Apis drives the report gene GFP in transgenic Drosophila in a pattern similar to the quadrant enhancer of vg of Drosophila.

(A,B) Drosophila vg-Q lacZ wing (A) and haltere (B) discs stained for LacZ (green) and Wg (red). Wg marks the D/V boundary. Drosophila vg-Q lacZ is expressed in non-D/V cells of the wing pouch, but is completely absent from the haltere discs. This transgenic line is reported by Kim et al. (1996). (C,D) Drosophila vg-Q GFP wing (C) and haltere (D) discs stained for GFP (green) and Wg (red). Note, the expression pattern is very similar to vg-Q lacZ. (E,F) Apis vg-Q GFP wing (E) and haltere (F) discs stained for GFP (green) and Wg (red). Note strong GFP expression in both wing and haltere discs. In both the discs, expression is limited to non-D/V cells of the pouch and in the presumptive hinge. Expression along the A/P boundary is lower, suggestive of quadrant expression pattern similar to the Drosophila vg-Q GFP. (G,H) vg-GAL4/ Apis vg-Q GFP; UAS-Ubx_Drosophila (G) and vg-GAL4/ Apis vg-Q GFP; UAS-Ubx_Apis (H) wing discs stained for GFP (green) and Wg (red). Note there is no change in the expression pattern of Apis vg-Q GFP. This is contrary to the severe repression observed for Drosophila vg-Q lacZ (Fig. 3J). (I,J) None of the two different mutant versions of Apis vg-Q GFP show any GFP staining in wing or haltere discs suggesting that mutating Adf-1 binding sites to MAD-binding sites may have resulted in complete loss of its activation during wing development. GFP that is seen in (I) is not nuclear and appears to be non-specific. In Apis vg-Q GFP^M1, Adf-1 binding site tggctgccgtcgcgat is replaced with MAD1-binding site (as in the Drosophila genome) gctgcccgccgc. In Apis vg-Q GFP^M2, Adf-1 binding site tggctgccgtcgcgat was replaced with MAD1-binding site (as in the Apis genome) gccgtcgc.