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. 2016 Jun 14;6:27876. doi: 10.1038/srep27876

Figure 2. Regulation of CYP3A29 expression via PXR.

Figure 2

(A) HepLi cells were transfected with 50 nM pcDNA-PXR expression plasmids or control vector for 24 h and treated with cecropin (B) (500 ng/ml) or PBS for 12 hours. CYP3A29/PXR mRNA and protein levels were detected by qRT-PCR and western blotting, respectively; * and ** P < 0.01 and P < 0.05, respectively. (B) HepLi cells were transfected with the PXR siRNA or the negative control for 24 hours and treated with cecropin (B) (500 ng/ml) or PBS for 12 hours. RNA interference efficiency was performed by western blotting(right). CYP3A29 mRNA levels were examined by qRT-PCR(feft). (C) HepLi cells were transfected with the RXR-α siRNA or the negative control for 24 h and then treated with cecropin B (500 ng/ml) or PBS for 12 h. RNA interference efficiency was determined by Western blotting (right). CYP3A29 and PXR mRNA levels were examined by qRT-PCR (left). The blot was cropped and the full-length blot is presented in Supplementary Fig. S2 (Fig. 2A–C).