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. 2016 Jun 14;6:27872. doi: 10.1038/srep27872

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(A,B) Fluorescence image and intensity of intracellular Ca²⁺ levels stained with Fluo 3-AM after plasma treatment for 20 s, 1 min and 3 min. (C) Measurement of the intracellular Ca²⁺ level by flow cytometry after plasma treatment for 20 s, 1 min and 3 min. M1 labeled the fluorescence positive cells with respect to the control group. (D,E) Percentage of fluorescence positive cells and fluorescence intensity after plasma treatment for 20 s, 1 min, and 3 min. n = 3, *indicates P < 0.05. **indicates P < 0.01.