| S. aureus |
| RN4220 |
Derived from NCTC8325-4;r-m+ |
39 |
| WT (RJ-2) |
CA-MRSA ST59 clinical isolate RJ-2 |
This study |
| Δagr
|
RJ-2 agr mutant; whole agr system deleted (from RNAIII to agrA) |
This study |
| Δhla
|
RJ-2 hla mutant |
This study |
| Δhla(pTXΔ16) |
RJ-2 hla mutant with pTXΔ16 |
This study |
| Δhla(pTXΔhla)
|
RJ-2 hla mutant with pTXΔhla
|
This study |
| Δpsmα |
RJ-2 psmα mutant; psmα1-4 operon deleted. |
This study |
| Δpsmα (pTXΔ16) |
RJ-2 psmα mutant with pTXΔ16 |
This study |
| Δpsmα (pTXΔpsmα) |
RJ-2 psmα mutant with pTXΔpsmα |
This study |
| Plasmids |
| pKOR1 |
cmR and ampR, temperature-sensitive (ts) vector for allelic replacement via lambda recombination and ccdB selection |
36 |
| pKOR1Δagr
|
ts vector for allelic replacement of agr in ST59 |
This study |
| pKOR1Δhla
|
ts vector for allelic replacement of hla in ST59 |
This study |
| pKOR1Δpsmα |
ts vector for allelic replacement of psmα in ST59 |
This study |
| pTXΔ16 |
Control plasmid for the pTXΔ plasmid series; lipase gene is deleted. |
8 |
| pTXΔpsmα |
pTXΔ plasmid containing the psmα1-4 genes of S. aureus; xylR is deleted in that plasmid series for constitutive gene expression. |
8 |
| pTXΔhla
|
pTXΔ plasmid containing the hla gene of S. aureus ST59; xylR is deleted in that plasmid series for constitutive gene expression. |
This study |
