Figure 3.

Expression of (A) COL1A1 and (B) MMP-8 and (C) hydroxyproline content in tibialis anterior tendons from rapamycin (RAPA) fed mice and age-matched old and adult controls. COL1A1 expression was unchanged with aging, but was decreased by 48% in tendons from RAPA-fed mice compared with age-matched controls. MMP-8 was expressed in extremely low levels in adult and old mice and was highly elevated in tendons from RAPA-fed mice. No age-associated changes in hydroxyproline concentration were seen, although tendons from old RAPA-fed mice trended toward decreased hydroxyproline content as compared with age-matched controls (p = .07). Data are presented as means ± SEM. *Significantly different from adult. ^#Significantly different from old.